Wednesday, October 30, 2019
Marketing Essay Example | Topics and Well Written Essays - 500 words - 3
Marketing - Essay Example Disney, being an international corporation, has the advantage of global exposure and its strength therefore is its vast experience. This experience is the key to success against small competitors who do not have the experience to match Disney in business. There are related opportunities too when it comes to Disney due to its long-standing relationship with a myriad of companies across the globe. Disney is therefore able to capitalize on new contracts, tenders and jobs without having to put in as much effort as any other competitor might put in. The bottom line here is that Disney has several strengths that have developed with time and have become a source of competitive advantage. The opportunities are the result of global strategic partnerships that the company has had over the past decades so that Disney has better opportunities than its competitors – always. The political issues are that how stable is the political government with the business and whether the government pol icy will either cause any changes in the tax payment. The environmental functions are regarding the surroundings and other things which compete in the business in the surroundings. The atmosphere is also covered in these issues.
Sunday, October 27, 2019
Effect of pH on Percent Oxygen Saturation of Haemaglobin
Effect of pH on Percent Oxygen Saturation of Haemaglobin Abstract The purpose of this lab was to determine the effect of changes in the pH and the partial pressure of oxygen on haemoglobin’s affinity for oxygen and percent oxygen saturation in the Bos taurus blood. At a lower pH (6.8) with a 21mmHg p50 value, it was found that the oxygen dissociation curve shifted to the right, which indicates that the Bohr Effect causes haemoglobin to lose its affinity for oxygen. This concludes that the increased carbon dioxide concentration forces haemoglobin to release its bound oxygen to the high metabolizing tissues (converting to deoxy-haemoglobin), but also forces the protein to bind more oxygen at the respiratory surfaces in order to transport enough oxygen to areas with a low oxygen partial pressure. At a higher pH (7.4), the oxygen dissociation curve shifted to the left with a 14mmHg p50 value since haemoglobin receives more oxygen, causing haemoglobin to have a higher affinity for oxygen and to remain as oxyhaemoglobin. Both sigmoid shaped curves contain a steep portion at 9.1mmHg and a plateau at 60mmHg. In conclusion, since the p50 value at pH 6.8 (21mmHg) is lower than the p50 value at pH 7.4 (14mmHg), this signifies that in an acidic environment, there is a higher partial pressure of oxygen in the environment as haemoglobin cannot bind the available oxygen as much due to its lowered affinity for oxygen. Introduction Haemoglobin is a specialized protein found inside the red blood cells of vertebrates that have developed organs for gaseous exchange as a substitute for simple diffusion. This respiratory pigment transports dissolved oxygen in the blood from the respiratory surface to the tissues through the circulatory system, and transports carbon dioxide back for excretion at the respiratory surface by exhalation. When one oxygen molecule binds to the iron containing heme group of the tetrameric-structured haemoglobinâ€â€which has four globulin subunits non-covalently linked to each other: two alpha- and two beta-polypeptide chainsâ€â€its allosteric property increases its affinity for the addition of the next three successive oxygen molecules (Fenton et al., 2015). A total of four oxygen molecules can bind to this protein; however changes in the partial pressure of oxygen at the gas exchange site can affect haemoglobin’s percent oxygen saturation and changes in pH can alter haemoglo bin’s affinity for oxygen (also known as the Bohr Effect). The magnitude of the Bohr Effect in haemoglobin results from the breaking of the salt bridges between the histidine residues when the protein changes between its two structures at equilibrium: oxyhaemoglobin and deoxyhaemoglobin (Kilmartin, 1974; Zheng et al., 2013). During the structural shift, the histidine residues will react with the sulfhydryl group of the cysteine that is found in the deoxyhaemoglobin (Okonjo et al., 2014). Consequently, this change in the structural conformation of cysteine leads to a reduced Bohr Effect (Okonjo et al., 2014). Since deoxyhaemoglobin has been altered at a chemical level, it will experience a minimized Bohr Effect while its affinity for oxygen increases (Riggs, 1988). The Bohr Effect states that in a high pH alkaline environment, protons are released when oxygen is attached to haemoglobin; but are not released when in an acidic environment (Riggs, 1988). To what extent did the variations of PO2 and pH levels affect haemoglobin’s percent oxygen saturation in the Bos taurus blood was examined as the main purpose in this lab and is depicted by an oxygen dissociation curve. The resultant sigmoidal (S) shape relates the partial pressure of oxygen and haemoglobin’s percent oxygen saturation at different pH levels. Given that the manometer PO2 is held constant at the specific mmHg value, it is predicted that the oxygen dissociation curve will shift to the right at a pH of 6.8 (which is lower than the normal pH value of 7.4) as there is an increase in carbon dioxide concentration. This indicates that a pH drop will decrease the percent oxygen saturation of haemoglobin, and hence, its affinity for oxygen. Conversely, it is estimated that the curve will shift to the left at a pH of 7.4 as there are less carbon dioxide molecules in an alkaline environment. It is also predicted that at P50, there will be a difference in the percent oxygen saturation of haemoglobin at the different pH levels and that the two sigmoidal curves of the oxygen dissociation graph will eventually meet at equilibrium past P50 due to the Bohr Effect. In relation, Hill conducted his experiment with a similar procedure on human muscle and haemoglobin and concluded that at a higher pH level (muscle haemoglobin at pH 9.2 and blood haemoglobin at pH 9.2), the curve shifted to the left while at a lower pH level (muscle haemoglobin at pH 7.0 and blood haemoglobin at pH 7.0), the curve shifted to the right due to the Bohr Effect (Hill, 1936). Materials and Methods This experiment was conducted based on the BIO202 lab manual; however, the procedures performed for the Bos taurus at pH 7.4 under section 2 was deviated from the instructions provided. The maximum vacuum pressure was set at 750 mmHg instead of the listed value of 700mmHg since vacuum did not provide enough pressure at 700mmHg to deoxygenate the blood appropriately. My lab partners, Isabel, Zainab and Dianne, performed the experimental tests on Bos taurus buffered at pH 7.4. We collected the data conducted at pH 6.8 from our colleagues: Kashamala, Kiran, Jing, and Seevin. Results Figure 1 This oxygen dissociation curve graph depicts a logarithmic regression that relates the effect of the partial pressure of oxygen on haemoglobin’s percent oxygen saturation at both pH levels in Bos taurus. The manometer reading was converted to the partial pressure of oxygen with equation a listed in the appendix. In addition, the percentage transmittance value obtained from the spectrophotometer was converted to the percent oxygen saturation of haemoglobin using the equations of the two logarithmic regression curves. The P50 value at pH 6.8 is ~21mmHg and at pH 7.4 is ~14mmHg. This value reveals the change in the partial pressure of oxygen at the different pH levels due to the Bohr Effect when 50% of haemoglobin has been saturated. In Figure 1, the effect of the partial pressure of oxygen on the percent oxygen saturation of haemoglobin illustrates a sigmoidal shape as the main trend at the pH of 6.8 and 7.4. As PO2 increases, haemoglobin’s oxygen saturation also increases until a maximum amount of oxygen molecules have bound to this blood pigment. There are two patterns in this oxygen dissociation graph: (1) the steep (beginning at 9.1mmHg) and (2) the plateau (~60mmHg). The steep section begins when PO2 first rises from 9.1mmHg and the percent saturation increases drastically. At pH 6.8, the steepness levels off at point three where PO2 is 30.1mmHg and the percent saturation is at 84.0826%. At pH 7.4, the steepness levels off at point two where PO2 is 30.1mmHg and the saturation is at 83.6601%. When haemoglobin has reached its maximum oxygen capacity, the curve relating PO2 and haemoglobin saturation plateaus and become theoretical. During this period, haemoglobin maintains its maximum oxygen saturation at the maximum PO2. At pH 6.8, the maximum PO2 of 156.12mmHg occurs when the vacuum is not used (the pressure remains at 0mmHg), which gives haemoglobin a maximum theoretical oxygen saturation of 102.612%. At pH 7.4, the maximum PO2 of 156.12mmHg gives haemoglobin a maximum oxygen saturation of 97.7172%, which is slightly lower than the value at pH 6.8. In terms of pH change (the Bohr Effect), the curve of the lower pH shifts to the right while the curve of the higher pH shifts to the left. However, the starting point for both of the sigmoidal-shaped curves is at PO2 of 9.1mmHg, where 6.9259% saturation is for pH 6.8 and 8.3333% saturation is for pH 7.4. Discussion The results support the hypothesis that the oxygen dissociation curve will shift to the right at the pH value of 6.8, showing that haemoglobin’s (Hb’s) percent oxygen saturation decreases (due to a lower affinity for oxygen), whereas the graph will shift to the left at the pH value of 7.4, demonstrating that haemoglobin’s percent oxygen saturation increases (due to a higher affinity for oxygen). The p50 values from the graph indicates that in a basic environment, there is a lower partial pressure of oxygen in the environment (14mmHg) when compared to an acidic environment (21mmHg) because haemoglobin is able to bind to more of the available oxygen due to its increased affinity for oxygen whereas at pH 6.8, haemoglobin is unable to bind to as much oxygen due to its decreased affinity for oxygen. The reason behind this lowered oxygen affinity is due to the Bohr Effect and the high concentration of carbon dioxide present in the area. Moreover, the amount of carbon d ioxide concentration present changes the pH, which directly affects the ability of haemoglobin to bind to oxygen and readily dissociate oxygen. When there is a high partial pressure of oxygen in an area where haemoglobin is present, the tetrameric protein’s percentage saturation of oxygen increases as well because there are more available oxygen molecules to bind to. Due to the cluster of oxygen molecules around this blood pigment, haemoglobins affinity for oxygen becomes tremendously improved due to its allosteric property, where the next three successive oxygen molecules are more readily bound after the first one has been attached. From this, since haemoglobin is able to transport more oxygen molecules from the respiratory surface, a high concentration of oxygen is released into the blood plasma to be absorbed by the surrounding tissues. This is why in Figure 1, the graphâ€â€comparing the effect of the partial pressure of oxygen on haemoglobin saturationâ€â€has an incredibly steep curve at the beginning to indicate that haemoglobin is able to pick up a lot of oxygen molecules. From the graph, at pH 6.8, there is a high partial pressure of carbon dioxide where water reacts with carbon dioxide, generating H+ ions that produce an acidic environment with a low pH (Riggs, 1988). This reduces haemoglobin’s affinity for oxygen, where haemoglobin subsequently converts into deoxyhaemoglobin, which is its alternate structural form. In this acidic environment, the tissues are at a high metabolic rate and need more oxygen molecules. So, according to the Bohr Effect, haemoglobin will bind to more oxygen at the respiratory surface and release its oxygen content more readily at the tissues (where there is a lowered PO2) in order to raise the pH level back to normal. In essence, the body raises the partial pressure of oxygen to match that of carbon dioxide so that a dynamic equilibrium is created to raise blood pH back to normal levels and reduce the acidity – where the rate of perfusion and ventilation is equal to each other. In terms of the pH at 7.4, ther e is low carbon dioxide partial pressure where the environment is more alkaline. The Bohr Effect prevents the transport of oxygen to the deoxygenated area, so that the partial pressure of oxygen also decreases in respect to the partial pressure of carbon dioxide in an attempt to decrease the pH level. However, if haemoglobin attaches onto oxygen, protons are released, which equals out the increase in oxygen with an increase in carbon dioxide. Thus, the increase in H+ is directly proportional to the increase in CO2 (Riggs, 1988). The two curves eventually meet together at one point, indicating that Bos taurus is attempting to maintain pH equilibrium, where pH 6.8 attempts to raise Hb saturation for a more alkaline environment and pH 7.4 attempts to reduce Hb saturation for a more acidic environment. However, the Bohr Effect presents an advantage to the body when it is subjected to an oxygen deficient environment during exercise, where there is lactic acid buildup, a drop in blood pH, and an increase in carbon dioxide and hydrogen concentrations. The formation of carbonic acid and H+ ions from the reaction between water and carbon dioxide produces an acidic environment, lowering the pH levels (Riggs, 1988). In these regions, the production of carbon dioxide from muscle cells inhibits haemoglobin’s affinity for oxygen in the bloodstream by binding to haemoglobin itself, forming carbamino haemoglobin. Carbamino haemoglobin brings the H+ protons generated along with the carbon dioxide molecules to the lungs, where it is expelled through exhalation (Riggs, 1988). When this happens, there is not enough oxygen present at the tissues, so the Bohr Effect sends haemoglobin to the tissues, which readily releases its oxygen content into the tissues because it can no longer hold onto the molecule as it travels further into the bloodstream. Eventually, the Bohr Effect helps to raise blood pH back up to normal levels when enduring anaerobic respiration since haemoglobin is continually releasing its bound oxygen molecules as its affinity decreases to be the rising carbon dioxide levels. It is beneficial for haemoglobin to have a low affinity for oxygen near the tissues to increase oxygen uptake by the tissues, but to have a high affinity for oxygen at respiratory surfaces so haemoglobin can transport as much oxygen as possible, and as far into the body as possible. Thus, altering the oxygen affinity of haemoglobin is the bodys way of using the Bohr Effect to its advantage to solve the problem of lacking oxygen since more oxygen is released with an increase in carbon dioxide levels. One limitation of the experiment involved the absence of a spectrophotometer and the instrumental error of the vacuum. The vacuum failed to serve its purpose at the 700mmHg level, so it had to be increased to the 750mmHg level for the procedure done for pH 7.4, which results in an unfair comparison for the data done for pH 6.8. In conclusion, when pH is low, haemoglobin has less affinity for oxygen (lower saturation) whereas when pH is high, haemoglobin has more affinity for oxygen (higher saturation). References Fenton, B., Dumont, B., and Owen, M. (2015). Respiratory Pigments: Hemoglobin and Hemocyanin. In Integrative Animal Biology (ed. S. Hinic-Frlog), pp. 300-301. Toronto, ON: Nelson Education Ltd. Hill, R. (1936). Oxygen Dissociation Curves of Muscle Haemoglobin. Proc. R. Soc. Lond. 120, 472-483. Kilmartin, J. V. (1974). THE ALKALINE BOHR EFFECT OF HUMAN HEMOGLOBIN.Annals of the New York Academy of Sciences. 241, 465-471. Okonjo, K. O., Olatunde, A. M., Fodeke, A. A. and Babalola, J. O. (2014). Bohr effect of human hemoglobin A: Magnitude of negative contributions determined by the equilibrium between two tertiary structures. Biophysical Chemistry. 190, 41-49. Riggs, A. F. (1988). The Bohr Effect. Annual Reviews Inc. 50, 181-204. Zheng, G., Schaefer, M. and Karpus, M. (2013). Hemoglobin Bohr Effects: Atomic Origin of the Histidine Residue Contributions. Biochemistry. 52, 8539-8555. Appendix Figure A1 This standard curve graph depicts a linear regression that relates the effect of percent transmittance on the percent saturation of haemoglobin at both pH levels in Bos taurus. Oxygenated haemolysate was exposed to the vacuum at a maximum pressure of 700mmHg for 5 minutes to become entirely deoxygenated, which was added to the oxygenated haemolysate. Different amounts of 100% oxygenated and 100% deoxygenated haemoglobin were combined to give five various saturation percentages. A percent transmittance reading was taken for each of the saturation percentages, using a spectrophotometer. Oxygen Dissociation Curve (Sample Calculation #1) The manometer reading values are converted to the partial pressure of oxygen with the formula: (Equation a), where D is the barometric pressure (measured in mmHg), W is the water vapor pressure (measured in mmHg), and M is the manometer reading (measured in mmHg). The temperature was 22 ºC and the barometric pressure was 763mmHg for the lab room. Manometer reading at 300mmHg: Therefore, the partial pressure of oxygen at a manometer reading of 300mmHg is 93.1mmHg. Oxygen Dissociation Curve (Sample Calculation #2) The regression line equation is used to convert the percent transmittance to the percent oxygen saturation of haemoglobin. At the pH 6.8: The regression line equation is, where the variable y represents the percent transmittance value and the variable x represents the percent oxygen saturation of haemoglobin. Partial pressure of oxygen at 93.1mmHg: Therefore, the percent oxygen saturation of haemoglobin is 98.0559% at the pH 6.8. At the pH 7.4: The regression line equation is, where the variable y represents the percent transmittance value and the variable x represents the percent oxygen saturation of haemoglobin. Partial pressure of oxygen at 93.1mmHg: Therefore, the percent oxygen saturation of haemoglobin is 94.7712% at the pH 7.4. Â
Friday, October 25, 2019
Persuasion Paper :: essays research papers
                                                  Matt B                                                       2/14/05                                                   PY129                         Persuasion Paper           Hello, woman who sits in the front of the class. I may not have your name, but I do have some unfortunate news for you. You should never go out with me. We all know it would have happened. Everybody knows what’s on a woman’s mind, and how could I not notice things like the way you stare at me in class, it’s just so distracting. I’m just trying to stop this very attractive problem before it’s too late. This paper will save you from years of therapy and gallons of Ben and Jerry’s cookie dough. Don’t feel bad, it’s not you, well actually it is; but you can still choose from the other 10 guys in our class who mysteriously all sit in a circle around you! I just don’t think things would work out between us. I believe that I have a pretty good idea of what kind of a person you are from being in your logic class and never talking to you once. Our close and personal relationship gives me insight to who you are, and why we aren’t right for each other. First, your too predictable and boring for me, second, your not willing to change, and third, I don’t think you could satisfy me.                     Variety is the spice of life! Mix things up a bit. You sit in the same seat every day, who does this? Don’t you ever wonder what it feels like to sit someplace else? I wouldn’t be able to stand that kind of compliancy. Another testament to your predictability, is the fact that you never have a story to tell about how your weekend was. This means either one of two things.           1) You do nothing on weekends. Your idea of fun is watching the Lifetime channel. You go to Six Flags and enjoy such rides as ‘Teacups,’ ‘Ferris wheel,’ and ‘Dirty bench by the hotdog stand.’      2) Just the opposite. Maybe you frequent S&M clubs or rob orphans on the weekends. Whatever it is, it probably makes the baby Jesus cry. You disgust me. So start enjoying the finer, more fun things in life. Until then, though, I just don’t think your ready for my kind of excitement.           You most likely do a variety of things, or have some qualities that are unattractive to me, and you are also unwilling to change them. I know this because of our many interactions together, where you have revealed to me your deepest secrets and habits.
Thursday, October 24, 2019
My Favorite Healthy Meal
I do, and it's a delicious turkey sub. It's my favorite meal because it's healthy, it fills me up, and best of all, it's scrumptious, all at the same time! This meal is healthy because it has Just about all the food groups you need in a healthy meal. It has meat, vegetables, dairy, and wheat. The reason the meat in my sub is turkey cold cut, is because turkey doesn't carry much fat in it. You get your vitamins and nutrients from the vegetables; and you get grains from the wheat bread.One of the advantages of dairy, is that dairy strengthens your bones. The ingredients needed are sliced, turkey cold meat, shredded lettuce, sliced cucumbers, sliced American cheese, low fat ranch, and a six-inch wheat bread that are used for subs. Before you start, wash your hands with warm water and soap. To start off, you have to get six-inches of wheat bread cut the sub from left to right. After you cut the bread open, take one slice of the turkey at a time, and starting from one end of the sub, line them up across the entire sub. Next, do the same thing with theAmerican cheese. After that, take the shredded lettuce and spread it across the sub on top of the meat and cheese. Next, get the sliced cucumbers and Just do the same as you do with the meat and cheese. In the next step, grab the low fat ranch and put one straight line of ranch on top of the vegetables, meat, and cheese. Lastly, close It up and enjoy your sub! In conclusion, I think this sub Is a great sub that I love to make and eat! It Is a great and healthy meal! I hope you get to make It and even more Importantly, Like It! Again, this Is a healthy, yet delicious meal!
Wednesday, October 23, 2019
Bullying Essay
Bullying is the use of force, or threat, to abuse, intimidate, or aggressively impose domination over others. Justifications and rationalizations for such behavior sometimes include differences of class, race, religion, gender, sexuality, appearance, behavior, or ability. (Wiki2013) This abuse like many others may not be known to us personally, but that doesn’t mean that we should ignore it. Sometimes given the fact that we are unaware of this abuse we don’t know how serious of a problem it can be. According to the organization against bullying; in recent years, a series of bullying- related suicides in the US, and around the world have drawn attention to the connection between bullying and suicide. Though many adults still see bullying as â€Å"just a part of being a kid†; it is a serious problem that leads to many negative effects for victims, including suicide. Bullying can be presented in different ways such as, physical bullying, emotional bullying, and even cyber bullying. Physical bullying involves hurting a person’s body or possessions. Physical bullying includes hitting, pinching, spitting, pushing, pulling, tripping, slapping and taking or breaking someone’s possessions. (Stop bullying.gov 2013) This physical aggression can occur almost anywhere; it can be at work, school, or even at home. For instance, children at school tend to hit their classmates when trying to get their attention. They normally don’t even call the individual by their name; they just hit, slap, or even kick the person to get his or her attention. This behavior is normally repeated with the same person on different occasions. Also, another thing that people do is find the weakest and least amiable person in their school. They gang up on him throw things at him, push him around and as though that wasn’t enough, they push their victims head into the toilet in the rest room. In addition, people at work also exert physical bullying. At times, at work there is a certain person that is the target of unoccupied individuals that are just seeking to humiliate others. This is carried about by actions such as, pulling away their colleagues chair when they are about to sit down. This is repeatedly done to make a mockery of that person. Bullies that perform these actions may not think about the harm being done, but many times the victims have scars from the physical abuse. Furthermore, Emotional bullying is another way of abusing people. Emotional bullying is the use of offensive/ obscene language used by bullies to degrade and demean their victims. (bullying.org) Persons are sometimes targeted because of the way they dress, walk, and talk or even because of their race or religion. Rude remarks are made ab out these persons by their bullies so often, that the victims tend to have low self -esteem and can even be depressed. For instance, I once saw a young lady in junior college that was pestered because she dressed in long skirts and dresses; that didn’t do any kind of drug and was always in mass. This person was mocked, insulted and called names because of her physical appearance and religious preference. In the same way persons who are homosexuals, are often made fun of. These persons are humiliated in every manner possible by bullies, also known in this case as homophobes. Furthermore, constantly spreading rumors about someone is also considered verbal abuse, because the victim is embarrassed by many persons over and over. These persons that are constantly molested at times also have suicidal thoughts. Therefore, we should all think very well before we insult, or â€Å"innocently†label people with offensive nicknames. Likewise, cyber bullying is just as serious as emotional/ verbal, and physical bullying. According to the organization against bullying, Cyber bullying is when a child, teen, adult, etc, is harassed, humiliated, embarrassed, threatened, or tormented using digital technology. This type of bullying like others is done to publicly embarrass a person. For example, a teenager who took a naked picture of herself for her boyfriend; the picture ended up being uploaded to the internet by her partner. All those who got to see this photo instantly commented making rude remarks and observations about the young lady. Her picture was shared with many other persons just to keep making fun of her unintelligent choice. Moreover, Cyber bullying can also be made through cell phones, via text message , or a call. Some hackers also tend to bully naà ¯ve people. They send text messages telling people you have won a prize, and to redeem the prize you must follow a set of instructions. This is done repeatedly too many persons by the same bully. No prize is ever obtained and in some cases the victims, end up losing money. Hence, we should all be careful about what we post on line or what things are sent via text message, because bullies are just waiting for their next victim. In conclusion, bullying is a form of aggressive behavior or threat towards another individual to intimidate, harm or cause discomfort; this is done repeatedly and intentionally. Bullying can be presented in different ways such as, physical bullying, emotional bullying, and even cyber bullying. As a matter of fact, the victims of this abuse normally don’t do anything to cause being bullied. Not to mention, these individuals don’t even defend themselves from their aggressor. With this in mind let’s try to be more civil and conscious individuals so that some day we may live in peace and harmony. References Bullying and Suicide – Bullying Statistics retrieved from: www.bullyingstatistics.org/content/bullying-and-suicide. Bullying retrieved from: wikipedia.org/wiki/Bullying Bullying Definition- StopBullying.gov retrieved from: www.stopbullying.gov
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